Immunomodulatory effects of Eimeria maxima surface antigen (EmSAG) as an IFN-γ inhibitory molecule on peripheral blood mononuclear cells (PBMCs) and T cell subsets in chickens

Table 1 Primer sequences used for quantitative real-time PCR

RNA target	Primer sequence (5' → 3')	Accession NO	Length of product
β-actin^a	GCCAACAGAGAGAAGATGACAC	NM_205518	140
β-actin^a	GTAACACCATCACCAGAGTCCA	NM_205518	140
IFN-γ^a	ATCATACTGAGCCAGATTGTTTCG	Y07922	140
IFN-γ^a	TCTTTCACCTTCTTCACGCCAT	Y07922	140
IL-2^a	TTCATCTCGAGCTCTACACACCAA	NM_204153	108
IL-2^a	TGTCATCTTCAGTTTCTTTCTTCAGAGT	NM_204153	108
TNF-α^b	AGTTCAGATGAGTTGCCCTTCCTG	XM_015294124	153
TNF-α^b	TTCAGAGCATCAACGCAAAAGGGA	XM_015294124	153
IL-4^a	AGCACTGCCACAAGAACCTG	NM_001007079	100
IL-4^a	CCTGCTGCCGTGGGACAT	NM_001007079	100
IL-10^a	CTTTGGCTGCCAGTCTGTGTC	NM_001004414	94
IL-10^a	GCTCTGCTGATGACTGGTGCT	NM_001004414	94
TGF-β1^a	GCCGACACGCAGTACACCAA	M31160	169
TGF-β1^a	TGCAGGCACGGACCACCAT	M31160	169
CTLA-4^a	CAAGATGGAGCGGATGTACC	NM_001040091	164
CTLA-4^a	TGGCTGAGATGATGATGCTG	NM_001040091	164

ISSN: 1297-9716